Oxyhemoglobin Method for Estimation of Hemoglobin
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Medically Reviewed
Hematology

Oxyhemoglobin Method for Estimation of Hemoglobin

Learn how to estimate hemoglobin using the oxyhemoglobin method. This guide covers the procedure, its similarities to the cyanmethemoglobin method, and its limitations regarding reliability and accuracy.

By Dayyal Dg.
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3D render of a medical background with blood cells.
3D render of a medical background with blood cells. Freepik / @kjpargeter

For the estimation of hemoglobin using the oxyhemoglobin method, a blood sample is combined with a weak ammonia solution. The absorbance of this mixture is then measured using either a photometer with a yellow-green filter or a spectrophotometer at 540 nanometers. The absorbance value of the test sample is compared with that of a standard solution.

This technique closely resembles the cyanmethemoglobin (hemoglobin-cyanide) method.

Despite being straightforward and rapid, the oxyhemoglobin method is less reliable than the cyanmethemoglobin method. This is due to the lack of a stable standard solution, the exclusion of hemoglobin derivatives other than oxyhemoglobin, and the rapid fading of the oxyhemoglobin solution's color.

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Dayyal Dg.. “Oxyhemoglobin Method for Estimation of Hemoglobin.” BioScience. BioScience ISSN 2521-5760, 16 July 2017. <https://www.bioscience.com.pk/en/topics/hematology/oxyhemoglobin-method-for-estimation-of-hemoglobin>. Dayyal Dg.. (2017, July 16). “Oxyhemoglobin Method for Estimation of Hemoglobin.” BioScience. ISSN 2521-5760. Retrieved June 26, 2024 from https://www.bioscience.com.pk/en/topics/hematology/oxyhemoglobin-method-for-estimation-of-hemoglobin Dayyal Dg.. “Oxyhemoglobin Method for Estimation of Hemoglobin.” BioScience. ISSN 2521-5760. https://www.bioscience.com.pk/en/topics/hematology/oxyhemoglobin-method-for-estimation-of-hemoglobin (accessed June 26, 2024).
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