Oxyhemoglobin Method for Estimation of Hemoglobin
Learn how to estimate hemoglobin using the oxyhemoglobin method. This guide covers the procedure, its similarities to the cyanmethemoglobin method, and its limitations regarding reliability and accuracy.

For the estimation of hemoglobin using the oxyhemoglobin method, a blood sample is combined with a weak ammonia solution. The absorbance of this mixture is then measured using either a photometer with a yellow-green filter or a spectrophotometer at 540 nanometers. The absorbance value of the test sample is compared with that of a standard solution.
This technique closely resembles the cyanmethemoglobin (hemoglobin-cyanide) method.
Despite being straightforward and rapid, the oxyhemoglobin method is less reliable than the cyanmethemoglobin method. This is due to the lack of a stable standard solution, the exclusion of hemoglobin derivatives other than oxyhemoglobin, and the rapid fading of the oxyhemoglobin solution's color.
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