Biochemical Analysis of Semen for Investigation of Infertility

Semen analysis involves the assessment of biochemical markers, as outlined in Table 1, to examine the secretions from various accessory structures. These markers encompass fructose, associated with seminal vesicles; zinc, citric acid, or acid phosphatase, linked to the prostate; and α-glucosidase or carnitine, indicative of epididymal contributions. This comprehensive approach allows for a nuanced evaluation of the diverse components contributing to semen composition, providing valuable insights into the functionality of the reproductive system.

Test for Fructose

The Resorcinol method serves as a means for detecting fructose within biological samples. In this analytical procedure, 5 ml of the resorcinol reagent is employed. This reagent is prepared by dissolving 50 mg of resorcinol in 33 ml of concentrated hydrochloric acid, followed by dilution up to 100 ml with distilled water. The resulting solution is then added to 0.5 ml of seminal fluid. Subsequently, the mixture is subjected to heat and brought to a boil. The emergence of a red-colored precipitate within a brief span of 30 seconds signifies the presence of fructose.

The absence of fructose in the test results points towards potential obstructions proximal to the seminal vesicles, which may manifest as either obstructed or absent vas deferens, or an absence of seminal vesicles altogether. In instances of azoospermia, the absence of fructose may be indicative of the obstruction of ejaculatory ducts or the absence of vas deferens. Conversely, the presence of fructose in cases of azoospermia suggests a failure of the testes to produce sperm. This nuanced interpretation allows for a more comprehensive understanding of the underlying factors contributing to the observed test results.