or go the traditional way

Login to your BIOSCIENCE. account and enjoy increased access to BIOSCIENCE. content, ideas and unlock downloads. A selection of top articles hand-picked by our editors available only to registered users.

Educational and corporate subscriptions give institutions and students unlimited access to the BIOSCIENCE. Archive, an essential tool for health-science and biology education and research.

Copyright © 2019 BIOSCIENCE PK, LLC. All rights reserved.

Dayyal Dg.

BIOSCIENCE member since 2018-08-01 17:27:24

Clinical laboratory professional specialized to external quality assessment (proficiency testing) schemes for Laboratory medicine and clinical pathology. Author/Writer/Blogger

Dayyal Dg.
RSS Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
Dayyal Dg.
Dayyal Dg. in Hemotology
Saturday, 29 July 2017 14:18

CAUSES OF ERRONEOUS RESULTS (INTERFERENCES CAUSING ABNORMAL RESULT) WITH HEMOTOLOGY ANALYZER

CAUSES OF ERRONEOUS RESULTS (INTERFERENCES CAUSING ABNORMAL RESULT)   These are listed in Table 809.1   Table 809.1 Causes of erroneous results with hematology analyzer Parameter Interfering factors   Erroneous increase Erroneous decrease 0. All parameters — Clotted sample 1. WBC count Nucleated red cells Large platelet clumps Unlysed red cells…

  • Hemotology
  • Hemotology Notes
  • Notes
  • Article
  • Laboratory Technique
  • Laboratory
  • Laboratory Equipment
  • Causes of Erroneous Results in Hemotology Analyzer
  • Automation in Lab
Read 2272 times
Dayyal Dg.
Dayyal Dg. in Hemotology
Saturday, 29 July 2017 12:54

PARAMETERS MEASURED BY HEMATOLOGY ANALYZERS

Parameters measured by hematology analyzers and their derivation are shown in Tables 808.1 and 808.2. Most automated hematology analyzers measure red cell count, red cell indices (mean cell volume, mean cell hemoglobin, mean cell hemoglobin concentration), hemoglobin, hematocrit, total leukocyte count, differential leukocyte count (three-part or five-part), and platelet count.

  • Hemotology
  • Hemotology Notes
  • Notes
  • Article
  • Laboratory
  • Laboratory Technique
  • Laboratory Equipment
  • Automation in Lab
  • How to
  • Parameters Measured by Hemotology Analyzer
Read 4857 times
Dayyal Dg.
Dayyal Dg. in Hemotology
Saturday, 29 July 2017 10:37

FLOW CYTOMETRY

FLOW CYTOMETRY   Flow cytometry is a procedure used for measuring multiple cellular and fluorescent properties of cells when they flow as a single cell suspension through a laser beam by a specialized instrument called as a flow cytometer. Flow cytometry can analyze numerous cells in a short time and…

  • Hemotology
  • Hemotology Notes
  • Notes
  • Article
  • Biomedical Engineering
  • Laboratory
  • Laboratory Equipment
  • Laboratory Technique
  • Flow Cytometer
  • Uses of Flow Cytometry
  • Automation in Lab
Read 1056 times
Dayyal Dg.
Dayyal Dg. in Hemotology
Saturday, 29 July 2017 10:09

TERMINOLOGIES USED IN FLOWCYTOMETRY

Fluorescence   A fluorochrome absorbs light energy and emits excess energy in the form of photon light (fluorescence). Fluorescence is the property of molecules to absorb light at one wavelength and emit light at a longer wavelength. The fluorescent dyes commonly used in flow cytometry are fluorescein isothiocyanate (FITC) and…

  • Terminologies Used in Flowcytometery
  • Hemotology
  • Hemotology Notes
  • Notes
  • Laboratory
  • Laboratory Technique
  • Laboratory Equipment
  • Informative
  • Flow Cytometer
  • Biomedical Engineering
  • Automation in Lab
Read 1279 times
Dayyal Dg.
Dayyal Dg. in Hemotology
Friday, 28 July 2017 12:59

COMMON APPLICATIONS OF FLOW CYTOMETRY IN HEMATOLOGY

Leukemias and lympomas: Immunophenotyping (evaluation of cell surface markers), diagnosis, detection of minimal residual disease, and to identify prognostically important subgroups. Paroxysmal nocturnal hemoglobinuria: Deficiency of CD 55 and CD 59. Hematopoietic stem cell transplantation: Enumeration of CD34+ stem cells. Feto-maternal hemorrhage: Detection and quantitation of foetal hemoglobin in maternal…

  • Hemotology
  • Hemotology Notes
  • Biomedical Engineering
  • Laboratory Equipment
  • Laboratory Technique
  • How to
  • Article
  • Notes
  • Flow Cytometer
  • Automation in Lab
Read 979 times
Dayyal Dg.
Dayyal Dg. in Hemotology
Friday, 28 July 2017 05:05

PLATELET AGGREGATION STUDIES

Platelet aggregation tests are carried out in specialized hematology laboratories if platelet dysfunction is suspected. These tests are usually indicated in patients presenting with mucocutaneous type of bleeding and in whom screening tests reveal normal platelet count, prolonged bleeding time, normal prothrombin time, and normal activated partial thromboplastin time.

  • Hemotology
  • Hemotology Notes
  • Notes
  • Article
  • Platelets
  • How to
  • Hemotological Tests
  • Blood Diseases and Disorders
  • Platelet Aggregation Test
  • Laboratory Test Procedure
  • Diseases and Disorders
Read 3779 times
Dayyal Dg.
Dayyal Dg. in Hemotology
Thursday, 27 July 2017 06:22

EXAMINATION OF BLOOD SMEAR

A blood smear is examined for:   Red cells: Morphology, immature forms, inclusion bodies, arrangement of cells. White cells: Differential count, abnormal or immature forms. Platelets: Adequacy, abnormal forms. Parasites: Malaria, filaria.   A peripheral blood smear has three parts: Head, body, and tail. Also read: PREPARATION OF BLOOD SMEAR…

  • Hemotology
  • Hemotology Notes
  • Notes
  • Article
  • How to
  • Hemotological Tests
  • Microbiology
  • Microbiology Notes
  • Examination of Blood Smear
  • Laboratory
  • Laboratory Technique
  • Laboratory Test Procedure
  • Diseases and Disorders
Read 4048 times
Dayyal Dg.
Dayyal Dg. in Hemotology
Wednesday, 26 July 2017 12:45

MORPHOLOGY OF PLATELETS

Platelets are small, 1-3 μm in diameter, purple structures with tiny irregular projections on surface. In blood films prepared from non-anticoagulated blood (i.e. direct fingerstick), they occur in clumps. If platelet count is done on automated blood cell counters using EDTA-anticoagulated blood sample, about 1% of persons show falsely low…

  • Hemotology
  • Hemotology Notes
  • Notes
  • Article
  • Microbiology
  • How to
  • Morphology of Platelets
  • Platelets
  • Platelets Morphology
  • Hemotological Tests
  • Blood Diseases and Disorders
  • Diseases and Disorders
  • Laboratory Test Procedure
Read 3308 times
Dayyal Dg.
Dayyal Dg. in Hemotology
Wednesday, 26 July 2017 11:28

NUMERICAL ABNORMALITIES OF LEUKOCYTES

For meaningful interpretation, absolute count of leukocytes should be reported. These are obtained as follows:   Absolute Leukocyte Count = Leukocyte% × Total Leukocyte Count/ml     Neutrophilia:   An absolute neutrophil count greater than 7500/μl is termed as neutrophilia or neutrophilic leukocytosis.   Causes   Acute bacterial infections: Abscess,…

  • Hemotology
  • Hemotology Notes
  • Notes
  • Article
  • How to
  • Numerical Abnormalities of Leukocytes
  • Blood Diseases and Disorders
  • Laboratory
  • Microbiology
  • Microbiology Notes
  • Hemotological Tests
  • Diseases and Disorders
  • Laboratory Test Procedure
Read 5788 times
Dayyal Dg.
Dayyal Dg. in Hemotology
Wednesday, 26 July 2017 08:33

WHITE BLOOD CELLS MORPHOLOGY

Approximate idea about total leukocyte count can be gained from the examination of the smear under high power objective (40× or 45×). A differential leukocyte count should be carried out. Abnormal appearing white cells are evaluated under oil-immersion objective.   Morphology of normal leukocytes (see Figure 800.1):   Polymorphonuclear neutrophil:…

  • Hemotology
  • Hemotology Notes
  • Notes
  • Article
  • How to
  • White Blood Cells Morphology
  • Hemotological Tests
  • Blood Diseases and Disorders
  • Microbiology
  • Diseases and Disorders
  • Laboratory Test Procedure
Read 15973 times
Page 10 of 18