Objective: To test organism's ability to tolerate various osmotic concentrations. Test Procedure1. Use a sterile loop or needle to inoculate broth tubes with different salt concentrations.2. Incubate at the optimum…
Objective: To test the organism's susceptibility to antibiotic penicillin. Test Procedure and Interpretation: See the Optochin Disc Test. Discovery of Penicillin The discovery of penicillin's antibiotic powers is attributed to…
Objective: To determine the organism's oxygen requirement. Test Procedure1. Inoculate 5 ml of BHI broth with your unknown organism and incubate overnight. We have found that broth cultures provide much…
Objective: To determine the presence of the oxidase enzymes (e.g. cytochrome c oxidase). Test Procedure and Interpretation1. Grow the culture on a BHI plate for 48 hours. Up to 7…
Objective: To test an organism's susceptibility to the chemical, optochin. Optochin susceptibility tests the fragility of the bacterial cell membrane. This test is mainly used to differentiate between Streptococcus pneumoniae…
Objective: To test an organism's susceptibility to the antibiotic novobiocin. Test Procedure1. Streak a BHI plate using a sterile cotton swab. Turn the plate 90 degrees and restreak with the…
Objective: To determine whether an organism is motile.   Test Procedure and Interpretation   1. Prepare a semisolid agar medium in a test tube. 2. Inoculate with a straight wire,…
Objective: To determine the ability of an organism to grow in 7.5% NaCl and ferment mannitol. Test Procedure1. Streak an MSA plate with a light line of inoculum from the pure…
Objective: Some pathogens are able to produce exoenzymes called hemolysins which lyse red blood cells and thus their action can be demonstrated on a blood agar plate. Test Procedure1. Using a sterile…
Objective: DNase mediates the hydrolysis of DNA. Methyl Green indicator is stable at pHs above 7.5 but becomes colorless at lower pHs. The hydrolysis of DNA in the agar by…
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