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05 Apr 2017
Objective: To test organism's ability to tolerate various osmotic concentrations. Test Procedure1. Use a sterile loop or needle to inoculate broth tubes with different salt concentrations.2. Incubate at the optimum…
05 Apr 2017
Objective: To test the organism's susceptibility to antibiotic penicillin. Test Procedure and Interpretation: See the Optochin Disc Test. Discovery of Penicillin The discovery of penicillin's antibiotic powers is attributed to…
05 Apr 2017
Objective: To determine the organism's oxygen requirement. Test Procedure1. Inoculate 5 ml of BHI broth with your unknown organism and incubate overnight. We have found that broth cultures provide much…
05 Apr 2017
Objective: To determine the presence of the oxidase enzymes (e.g. cytochrome c oxidase). Test Procedure and Interpretation1. Grow the culture on a BHI plate for 48 hours. Up to 7…
05 Apr 2017
Objective: To test an organism's susceptibility to the chemical, optochin. Optochin susceptibility tests the fragility of the bacterial cell membrane. This test is mainly used to differentiate between Streptococcus pneumoniae…
05 Apr 2017
Objective: To test an organism's susceptibility to the antibiotic novobiocin. Test Procedure1. Streak a BHI plate using a sterile cotton swab. Turn the plate 90 degrees and restreak with the…
05 Apr 2017
Objective: To determine whether an organism is motile.   Test Procedure and Interpretation   1. Prepare a semisolid agar medium in a test tube. 2. Inoculate with a straight wire,…
05 Apr 2017
Objective: To determine the ability of an organism to grow in 7.5% NaCl and ferment mannitol. Test Procedure1. Streak an MSA plate with a light line of inoculum from the pure…
05 Apr 2017
Objective: Some pathogens are able to produce exoenzymes called hemolysins which lyse red blood cells and thus their action can be demonstrated on a blood agar plate. Test Procedure1. Using a sterile…
05 Apr 2017
Objective: DNase mediates the hydrolysis of DNA. Methyl Green indicator is stable at pHs above 7.5 but becomes colorless at lower pHs. The hydrolysis of DNA in the agar by…
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