Medically reviewed and approved by a board-certified member
Microbiology

MOTILITY TEST

Dayyal Dg.
By Dayyal Dg.
Published: Tuesday, 04 April 2017
Updated: Tuesday, 04 April 2017 20:48
BS
Login to get unlimited free access
Be the first to comment!
Motility Determination in Bacteria Amrita University
Objective: To determine whether an organism is motile.
 
Test Procedure and Interpretation
 
1. Prepare a semisolid agar medium in a test tube.
2. Inoculate with a straight wire, making a single stab down the center of the tube to about half the depth of the medium.
3. Incubate under the conditions favoring motility.
4. Incubate at 37°C
5. Examine at intervals, e.g. after 6 h, and 1 and 2 days (depends on generation time of bacteria) . Freshly prepared medium containing 1% glucose can be used for motility tests on anaerobes.
 
Results: Hold the tube up to the light and look at the stab line to determine motility.
 
Non-motile bacteria generally give growths that are confined to the stab-line, have sharply defined margins and leave the surrounding medium clearly transparent.
Motile Bacteria typically give diffuse, hazy growths that spread throughout the medium rendering it slightly opaque.
Was this page helpful?
(0 votes)
MOTILITY TEST
End of the article