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Hemotology

PLATELET GLYCOPROTEIN ANALYSIS

Dayyal Dg.
By Dayyal Dg.
Published: Thursday, 03 August 2017
Updated: Sunday, 06 August 2017 07:05
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This is done by flow cytometric analysis for detection of lack of GpIb/IX in Bernanrd Soulier syndrome (deficiency of CD42), and lack of GpIIb/IIIa in Glanzmann’s thrombasthenia (deficiency of CD41, CD61).
 
What is the best protocol for platelet glycoprotein (GPIIb/IIIa) analysis using flow cytometry?
 
Fresh platelets should always be used. Storing platelets dramatically changes the level of transmembrane proteins. The best way is to follow one of standardized protocols defined in: Immunophenotypic analysis of platelets. Krueger LA, Barnard MR, Frelinger AL 3rd, Furman MI, Michelson AD.Curr Protoc Cytom. 2002 Feb;Chapter 6:Unit 6.10
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  • Posted by Dayyal Dg.
PLATELET GLYCOPROTEIN ANALYSIS
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