ABO GROUPING AND Rh D GROUPING
There are two methods for ABO grouping:
- Cell grouping (forward grouping): Red cells are tested for the presence of A and B antigens employing known specific anti-A and anti-B (and sometimes anti-A, B) sera.
- Serum grouping (reverse grouping): Serum is tested for the presence of anti-A and anti-B antibodies by employing known group A and group B reagent red cells.
Both cell and serum grouping should be done since each test acts as a check on the other.
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