ABO GROUPING AND Rh D GROUPING

ABO Grouping

There are two methods for ABO grouping:

• Cell grouping (forward grouping): Red cells are tested for the presence of A and B antigens employing known specific anti-A and anti-B (and sometimes anti-A, B) sera.
• Serum grouping (reverse grouping): Serum is tested for the presence of anti-A and anti-B antibodies by employing known group A and group B reagent red cells.

Both cell and serum grouping should be done since each test acts as a check on the other.

There are three methods for blood grouping: slide, tube and microplate. Tube and microplate methods are better and are employed in blood banks.

Further Reading:

• DETERMINATION OF BLOOD GROUP BY SLIDE METHOD
• DETERMINATION OF BLOOD GROUP BY TUBE METHOD
• MICROPLATE TECHNIQUE FOR RH D GROUPING
• FALSE REACTION IN ABO GROUPING
• Rh D GROUPING METHOD