This is done by flow cytometric analysis for detection of lack of GpIb/IX in Bernanrd Soulier syndrome (deficiency of CD42), and lack of GpIIb/IIIa in Glanzmann’s thrombasthenia (deficiency of CD41,…
FDPs are fragments produced by proteolytic digestion of fibrinogen or fibrin by plasmin. For determination of FDPs, blood is collected in a tube containing thrombin (to remove all fibrinogen by…
Automated hematology analyzers work on different principles: Electrical impedance Light scatter Fluorescence Light absorption Electrical conductivity. Most analyzers are based on a combination of different principles. (1)…
AUTOMATED HEMOTOLOGY ANALYZER Automation is a process of replacement of tasks hitherto performed by humans by computerized methods. Until recently, hematological tests were performed only by manual methods. These methods,…
CAUSES OF ERRONEOUS RESULTS (INTERFERENCES CAUSING ABNORMAL RESULT) These are listed in Table 809.1 Table 809.1 Causes of erroneous results with hematology analyzer Parameter Interfering factors Erroneous increase…
Parameters measured by hematology analyzers and their derivation are shown in Tables 808.1 and 808.2. Most automated hematology analyzers measure red cell count, red cell indices (mean cell volume, mean…
FLOW CYTOMETRY Flow cytometry is a procedure used for measuring multiple cellular and fluorescent properties of cells when they flow as a single cell suspension through a laser beam…
Fluorescence A fluorochrome absorbs light energy and emits excess energy in the form of photon light (fluorescence). Fluorescence is the property of molecules to absorb light at one wavelength…
Leukemias and lympomas: Immunophenotyping (evaluation of cell surface markers), diagnosis, detection of minimal residual disease, and to identify prognostically important subgroups. Paroxysmal nocturnal hemoglobinuria: Deficiency of CD 55 and CD…
