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Objective: To determine the ability of an organism to produce proteolytic-like enzymes (gelatinases) which break down gelatin. Gelatinase destroys (hydrolyzes) the gel and causes its collapse and liquefaction. Test Procedure1.…
Published in
Microbiology
Objective: To determine the ability of an organism to hydrolyze the glycoside esculin to esculetin and glucose. Esculetin reacts with iron to form a dark brown to black complex. The…
Published in
Microbiology
Objective: To test for the presence of the enzyme catalase. Test Procedure1. If the plate is refrigerated, it should be allowed to warm up to room temperature and then incubated…
Published in
Microbiology
Total leukocyte count (TLC) refers to the number of white blood cells in 1 μl of blood (or in 1 liter of blood if the result is expressed in SI…
Published in
Microbiology
Platelets Platelets, also called "thrombocytes", are blood cells whose function (along with the coagulation factors) is to stop bleeding. Platelets have no nucleus: they are fragments of cytoplasm which are…
Published in
Microbiology
Size: 6.7 to 7.7 μ in diameter. Cytoplasm: Pink in color. The mature red blood cell is a nonnucleated, round, biconcave cell. Erythropoiesis: The main function of the red blood…
Published in
Microbiology
Friday, 29 April 2016 18:53
Difference Between Total Leukocyte Count and Differential Leukocyte Count!
Written by Dayyal Dg.
Variations of leukocyte count occur in many infectious, hematologic, inflammatory, and neoplastic diseases. Therefore, laboratory evaluation of almost all patients begins with the examination of the patient’s blood for total…
Published in
Microbiology
Sunday, 01 May 2016 06:30
WHO Hemoglobin Color Scale for the Estimation of Hemoglobin
Written by Dayyal Dg.
This method conceived and formulated by Stott and Lewis in 1995. This method is much similar in principle to the now outdated Tallqvist method. Positive technical changes have been made…
Published in
Microbiology
Monday, 09 May 2016 17:12
Erythrocyte Sedimentation Rate (ESR): Significance, Stages, Methods, Interpretation and Reference Ranges
Written by Dayyal Dg.
The erythrocyte sedimentation rate (ESR) measures the rate of settling (sedimentation) of erythrocytes in anticoagulated whole blood. Anticoagulated blood is allowed to stand in a glass tube for 1 hour…
Published in
Microbiology