Displaying items by tag: Platelet Glycoprotein Analysis

Home
Article Index

Language

Detection of platelet specific IgG antibodies to platelet glycoproteins (GP) IIb/IIIa, Ia/IIa, Ib/IX, IV or HLA class I (GP IIb/IIIa in this example) by MACE assay. Washed platelets were first incubated with human plasma (containing anti-platelet GP IIb/IIIa in this sample). The antibody-sensitized platelets were then lysed and the supernatant was added to a microplate precoated with murine monoclonal antibodies (MoAb) specific to platelet GP or HLA class I. Any human IgG bound to the GP or HLA class I antigen were detected with a alkaline phosphatase (ALP) conjugated antihuman IgG and substrate solution.

PLATELET GLYCOPROTEIN ANALYSIS

03 Aug 2017

This is done by flow cytometric analysis for detection of lack of GpIb/IX in Bernanrd Soulier syndrome (deficiency of CD42), and lack of GpIIb/IIIa in Glanzmann’s thrombasthenia (deficiency of CD41, CD61).

What is the best protocol for platelet glycoprotein (GPIIb/IIIa) analysis using flow cytometry?

Fresh platelets should always be used. Storing platelets dramatically changes the level of transmembrane proteins. The best way is to follow one of standardized protocols defined in: Immunophenotypic analysis of platelets. Krueger LA, Barnard MR, Frelinger AL 3rd, Furman MI, Michelson AD.Curr Protoc Cytom. 2002 Feb;Chapter 6:Unit 6.10.