Displaying articles by tag: Hemotology Notes

Dayyal Dg. in Hemotology
Tuesday, 29 January 2019 06:23

Adverse Effects of Transfusion

Blood transfusion is a life-saving procedure in an appropriate setting and there are no side-effects in the majority of cases. However, it is a potentially harmful procedure and every recipient of transfusion is at risk of an adverse reaction. It should be prescribed only if there is a definite clinical…

  • Hemotology
  • Hemotology Notes
  • Article
  • Blood Transfusion Reaction
  • Blood Transfusion
  • Massive Blood Transfusion Complications
  • Jehovah Witness Blood Transfusion
  • Blood Infusion
  • Blood Transfusion Definition
  • Blood Transfusion Reasons
  • Blood Transfusion Meaning
  • Blood Transfusion Procedure
  • Complications of Blood Transfusion
Read 4203 times
Dayyal Dg. in Clinical Pathology
Thursday, 03 August 2017 13:15

LABORATORY TESTS IN PORPHYRIAS

Porphyrias (from Greek porphura meaning purple pigment; the name is probably derived from purple discoloration of some body fluids during the attack) are a heterogeneous group of rare disorders resulting from disturbance in the heme biosynthetic pathway leading to the abnormal accumulations of red and purple pigments called as porphyrins…

  • Hemotology Notes
  • Hemotology
  • Notes
  • Article
  • Laboratory
  • Blood Diseases and Disorders
  • Laboratory Tests in Porphyrias
  • Hemotological Tests
  • Pathology
  • Pathology Notes
  • Clinical Pathology
  • Diseases and Disorders
Read 3236 times
Dayyal Dg. in Hemotology
Thursday, 03 August 2017 11:55

PLATELET GLYCOPROTEIN ANALYSIS

This is done by flow cytometric analysis for detection of lack of GpIb/IX in Bernanrd Soulier syndrome (deficiency of CD42), and lack of GpIIb/IIIa in Glanzmann’s thrombasthenia (deficiency of CD41, CD61).   What is the best protocol for platelet glycoprotein (GPIIb/IIIa) analysis using flow cytometry?   Fresh platelets should always…

  • Hemotology
  • Hemotology Notes
  • Notes
  • Article
  • How to
  • Hemotological Tests
  • Blood Diseases and Disorders
  • Platelet Glycoprotein Analysis
  • Laboratory Test in Bleeding Disorder
  • Diseases and Disorders
  • Laboratory Test Procedure
Read 1126 times
Dayyal Dg. in Hemotology
Thursday, 03 August 2017 11:33

TEST FOR D-DIMER

D-dimer is derived from the breakdown of fibrin by plasmin and D-dimer test is used to evaluate fibrin degradation. Blood sample can be either serum or plasma. Latex or polystyrene microparticles coated with monoclonal antibody to D-dimer are mixed with patient’s sample and observed for microparticle agglutination. As the particle…

  • Hemotology
  • Hemotology Notes
  • Notes
  • Article
  • How to
  • Laboratory Test in Bleeding Disorder
  • Hemotological Tests
  • Blood Diseases and Disorders
  • DDimer Test Procedure
  • Diseases and Disorders
  • Laboratory Test Procedure
Read 4211 times
Dayyal Dg. in Hemotology
Thursday, 03 August 2017 08:02

TEST FOR FIBRINOGEN/FIBRIN DEGRADATION PRODUCTS (FDPs)

FDPs are fragments produced by proteolytic digestion of fibrinogen or fibrin by plasmin. For determination of FDPs, blood is collected in a tube containing thrombin (to remove all fibrinogen by converting it into a clot) and soybean trypsin inhibitor (to inhibit plasmin and thus prevent in vitro breakdown of fibrin).…

  • Hemotology
  • Hemotology Notes
  • Notes
  • Article
  • How to
  • Test for Fibrinogen
  • Test for Fibrin Degradation Products
  • Laboratory Test in Bleeding Disorder
  • Hemotological Tests
  • Blood Diseases and Disorders
  • Laboratory Test Procedure
  • Diseases and Disorders
Read 4572 times
Dayyal Dg. in Hemotology
Monday, 31 July 2017 12:06

PRINCIPLES OF WORKING OF AUTOMATED HEMOTOLOGY ANALYZER

Automated hematology analyzers work on different principles:   Electrical impedance Light scatter Fluorescence Light absorption Electrical conductivity.   Most analyzers are based on a combination of different principles.   (1) Electrical impedance: This is the classic and timetested technology for counting cellular elements of blood. As this method of cell…

  • Hemotology
  • Hemotology Notes
  • Notes
  • Article
  • Laboratory
  • Laboratory Technique
  • Laboratory Equipment
  • Automation in Lab
  • Biomedical Engineering
  • Principles of Working of Automated Hemotology Analyzer
Read 27567 times
Dayyal Dg. in Hemotology
Sunday, 30 July 2017 13:20

ADVANTAGES AND DISADVANTAGES OF AUTOMATED HEMOTOLOGY ANALYZER

AUTOMATED HEMOTOLOGY ANALYZER Automation is a process of replacement of tasks hitherto performed by humans by computerized methods. Until recently, hematological tests were performed only by manual methods. These methods, though still performed in many peripheral laboratories, are laborintensive, and involve use of hemocytometers (counting chambers), centrifuges, Wintrobe tubes, photometers,…

  • Hemotology
  • Hemotology Notes
  • Notes
  • Article
  • Advantages and Disadvantages of Automated Hemotology Analyzer
  • Automation in Lab
  • Laboratory
  • Laboratory Technique
  • Laboratory Equipment
Read 19440 times
Dayyal Dg. in Hemotology
Saturday, 29 July 2017 14:18

CAUSES OF ERRONEOUS RESULTS (INTERFERENCES CAUSING ABNORMAL RESULT) WITH HEMOTOLOGY ANALYZER

CAUSES OF ERRONEOUS RESULTS (INTERFERENCES CAUSING ABNORMAL RESULT)   These are listed in Table 809.1   Table 809.1 Causes of erroneous results with hematology analyzer Parameter Interfering factors   Erroneous increase Erroneous decrease 0. All parameters — Clotted sample 1. WBC count Nucleated red cells Large platelet clumps Unlysed red cells…

  • Hemotology
  • Hemotology Notes
  • Notes
  • Article
  • Laboratory Technique
  • Laboratory
  • Laboratory Equipment
  • Causes of Erroneous Results in Hemotology Analyzer
  • Automation in Lab
Read 4163 times
Dayyal Dg. in Hemotology
Saturday, 29 July 2017 12:54

PARAMETERS MEASURED BY HEMATOLOGY ANALYZERS

Parameters measured by hematology analyzers and their derivation are shown in Tables 808.1 and 808.2. Most automated hematology analyzers measure red cell count, red cell indices (mean cell volume, mean cell hemoglobin, mean cell hemoglobin concentration), hemoglobin, hematocrit, total leukocyte count, differential leukocyte count (three-part or five-part), and platelet count.

  • Hemotology
  • Hemotology Notes
  • Notes
  • Article
  • Laboratory
  • Laboratory Technique
  • Laboratory Equipment
  • Automation in Lab
  • How to
  • Parameters Measured by Hemotology Analyzer
Read 9156 times
Dayyal Dg. in Hemotology
Saturday, 29 July 2017 10:37

FLOW CYTOMETRY

FLOW CYTOMETRY   Flow cytometry is a procedure used for measuring multiple cellular and fluorescent properties of cells when they flow as a single cell suspension through a laser beam by a specialized instrument called as a flow cytometer. Flow cytometry can analyze numerous cells in a short time and…

  • Hemotology
  • Hemotology Notes
  • Notes
  • Article
  • Biomedical Engineering
  • Laboratory
  • Laboratory Equipment
  • Laboratory Technique
  • Flow Cytometer
  • Uses of Flow Cytometry
  • Automation in Lab
Read 1650 times
Dayyal Dg. in Hemotology
Saturday, 29 July 2017 10:09

TERMINOLOGIES USED IN FLOWCYTOMETRY

Fluorescence   A fluorochrome absorbs light energy and emits excess energy in the form of photon light (fluorescence). Fluorescence is the property of molecules to absorb light at one wavelength and emit light at a longer wavelength. The fluorescent dyes commonly used in flow cytometry are fluorescein isothiocyanate (FITC) and…

  • Terminologies Used in Flowcytometery
  • Hemotology
  • Hemotology Notes
  • Notes
  • Laboratory
  • Laboratory Technique
  • Laboratory Equipment
  • Informative
  • Flow Cytometer
  • Biomedical Engineering
  • Automation in Lab
Read 1960 times
Dayyal Dg. in Hemotology
Friday, 28 July 2017 12:59

COMMON APPLICATIONS OF FLOW CYTOMETRY IN HEMATOLOGY

Leukemias and lympomas: Immunophenotyping (evaluation of cell surface markers), diagnosis, detection of minimal residual disease, and to identify prognostically important subgroups. Paroxysmal nocturnal hemoglobinuria: Deficiency of CD 55 and CD 59. Hematopoietic stem cell transplantation: Enumeration of CD34+ stem cells. Feto-maternal hemorrhage: Detection and quantitation of foetal hemoglobin in maternal…

  • Hemotology
  • Hemotology Notes
  • Biomedical Engineering
  • Laboratory Equipment
  • Laboratory Technique
  • How to
  • Article
  • Notes
  • Flow Cytometer
  • Automation in Lab
Read 1349 times
Dayyal Dg. in Hemotology
Friday, 28 July 2017 05:05

Platelet Aggregation Studies

Platelet aggregation tests are carried out in specialized hematology laboratories if platelet dysfunction is suspected. These tests are usually indicated in patients presenting with mucocutaneous type of bleeding and in whom screening tests reveal normal platelet count, prolonged bleeding time, normal prothrombin time, and normal activated partial thromboplastin time.

  • Hemotology
  • Hemotology Notes
  • Notes
  • Article
  • Platelets
  • How to
  • Hemotological Tests
  • Blood Diseases and Disorders
  • Platelet Aggregation Test
  • Laboratory Test Procedure
  • Diseases and Disorders
Read 8065 times
Dayyal Dg. in Hemotology
Thursday, 27 July 2017 06:22

EXAMINATION OF BLOOD SMEAR

A blood smear is examined for:   Red cells: Morphology, immature forms, inclusion bodies, arrangement of cells. White cells: Differential count, abnormal or immature forms. Platelets: Adequacy, abnormal forms. Parasites: Malaria, filaria.   A peripheral blood smear has three parts: Head, body, and tail. Also read: PREPARATION OF BLOOD SMEAR…

  • Hemotology
  • Hemotology Notes
  • Notes
  • Article
  • How to
  • Hemotological Tests
  • Microbiology
  • Microbiology Notes
  • Examination of Blood Smear
  • Laboratory
  • Laboratory Technique
  • Laboratory Test Procedure
  • Diseases and Disorders
Read 8019 times
Dayyal Dg. in Hemotology
Wednesday, 26 July 2017 12:45

MORPHOLOGY OF PLATELETS

Platelets are small, 1-3 μm in diameter, purple structures with tiny irregular projections on surface. In blood films prepared from non-anticoagulated blood (i.e. direct fingerstick), they occur in clumps. If platelet count is done on automated blood cell counters using EDTA-anticoagulated blood sample, about 1% of persons show falsely low…

  • Hemotology
  • Hemotology Notes
  • Notes
  • Article
  • Microbiology
  • How to
  • Morphology of Platelets
  • Platelets
  • Platelets Morphology
  • Hemotological Tests
  • Blood Diseases and Disorders
  • Diseases and Disorders
  • Laboratory Test Procedure
Read 5692 times
Page 1 of 3