Displaying articles by tag: Blood Banking and Transfusion Medicine

Dayyal Dg. in Hemotology
Friday, 21 July 2017 06:48

ABO GROUPING AND Rh D GROUPING

ABO Grouping There are two methods for ABO grouping: Cell grouping (forward grouping): Red cells are tested for the presence of A and B antigens employing known specific anti-A and anti-B (and sometimes anti-A, B) sera. Serum grouping (reverse grouping): Serum is tested for the presence of anti-A and anti-B…

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Dayyal Dg. in Hemotology
Friday, 21 July 2017 06:19

FALSE REACTION IN ABO GROUPING

Autoagglutination: Presence of IgM autoantibodies reactive at room temperature in patient’s serum can lead to autoagglutination. If autocontrol is not used, blood group in such a case will be wrongly typed as AB. Therefore, for correct result, if autocontrol is also showing agglutination, cell grouping should be repeated after washing…

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  • Blood Banking and Transfusion Medicine
  • False Reaction in ABO Grouping
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Dayyal Dg. in Hemotology
Friday, 21 July 2017 05:47

Rh D GROUPING METHOD

D antigen is the most immunogenic after ABO antigens and therefore red cells are routinely tested for D. Individuals are called as Rh-positive or Rh-negative depending on presence or absence of D antigen on their red cells. Following transfusion of Rhpositive blood to Rh-negative persons, 70% of them will develop…

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Dayyal Dg. in Hemotology
Friday, 21 July 2017 05:28

Microplate Technique for Rh D Grouping

Microplate is a polystyrene plate consisting of 96 micro wells of either U- or V-shape. Grouping is carried out in micro wells. This method is sensitive and ideal for large number of samples (see Figure 787.1).   Further reading: Rh D GROUPING METHOD

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  • Blood Banking and Transfusion Medicine
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Dayyal Dg. in Hemotology
Wednesday, 19 July 2017 08:46

DETERMINATION OF BLOOD GROUP BY SLIDE METHOD

Principle Red cells from the specimen are reacted with reagent antisera (anti-A and anti-B). Agglutination of red cells indicates presence of corresponding antigen (agglutinogen) on red cells. Specimen Capillary blood from finger prick, or venous blood collected in EDTA anticoagulant. Reagents ABO antisera: See box 786.1 and Figure 786.1. Box…

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Dayyal Dg. in Hemotology
Wednesday, 19 July 2017 07:53

DETERMINATION OF BLOOD GROUP BY TUBE METHOD

Test tube method is more reliable than slide test, but takes longer time and more equipment. For cell grouping, patient’s saline-washed red cells are mixed with known antiserum in a test tube; the mixture is incubated at room temperature, and centrifuged. For serum grouping, patient’s serum is mixed with reagent…

  • Hemotology
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  • Estimation of Blood Group by Tube Method
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  • Blood Bank
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  • Blood Banking and Transfusion Medicine
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Chetan Dg. in Downloads
Sunday, 28 May 2017 14:35

Blood Banking and Transfusion Medicine

Description: Ever since the discovery of blood types early in the last century, transfusion medicine has evolved at a breakneck pace. This second edition of Blood Banking and Transfusion Medicine is exactly what you need to keep up. It combines scientific foundations with today's most practical approaches to the specialty.…

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