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ABO Grouping

There are two methods for ABO grouping:
 
  • Cell grouping (forward grouping): Red cells are tested for the presence of A and B antigens employing known specific anti-A and anti-B (and sometimes anti-A, B) sera.
  • Serum grouping (reverse grouping): Serum is tested for the presence of anti-A and anti-B antibodies by employing known group A and group B reagent red cells.

Both cell and serum grouping should be done since each test acts as a check on the other.
 
There are three methods for blood grouping: slide, tube and microplate. Tube and microplate methods are better and are employed in blood banks.
 
Further Reading:
 

Dictionary:

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